LOCUS       40924_37753        6514 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRS304 stem-vYFPdel-5-1.2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Belmont BJ, Niles JC.
  TITLE     Inducible Control of Subcellular RNA Localization Using a Synthetic 
            Protein-RNA Aptamer Interaction
  JOURNAL   PLoS ONE 7 (10), E46868 (2012)
  PUBMED    23056498
REFERENCE   2  (bases 1 to 6514)
  AUTHORS   Belmont BJ, Niles JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2012) Biological Engineering, Massachusetts 
            Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139, 
            USA
REFERENCE   3  (bases 1 to 6514)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6514)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2012"; volume: "7"; issue: "10"; pages: "E46868"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-SEP-2012) Biological Engineering, Massachusetts Institute of 
            Technology, 77 Massachusetts Ave, Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     17..33
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             646..1317
                     /codon_start=1
                     /label=(3-F)Tyr-EGFP
                     /note="(3-F)Tyr-EGFP is a basic (constitutively
                     fluorescent) green fluorescent protein published in 2004, 
                     derived from Aequorea victoria."
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     ICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEF
                     VT"
     primer_bind     complement(1718..1734)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(2493..3655)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the
                     autonomously replicating sequence ARS1/ARS416"
     promoter        complement(4169..4270)
                     /label=TRP1 promoter
     promoter        4376..4480
                     /label=AmpR promoter
     CDS             4481..5338
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5512..6100
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6388..6409
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6424..6454
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6462..6478
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6486..6502
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcccagtcac gacgttgtaa aacgacggcc agtgctacaa tcgcggccgc ataggtatcg
       61 atcgtcaccc tgcagggtga cggatcgatc cgtcacccgc atattacata taatacatat
      121 cacataggaa gcaacaggcg cgttggactt ttaattttcg aggaccgcga atccttacat
      181 cacacccaat cccccacaag tgatccccca cacaccatag cttcaaaatg tttctactcc
      241 ttttttactc ttccagattt tctcggactc cgcgcatcgc cgtaccactt caaaacaccc
      301 aagcacagca tactaaattt cccctctttc ttcctctagg gtgtcgttaa ttacccgtac
      361 taaaggtttg gaaaagaaaa aagagaccgc ctcgtttctt tttcttcgtc gaaaaaggca
      421 ataaaaattt ttatcacgtt tctttttctt gaaaattttt ttttttgatt tttttctctt
      481 tcgatgacct cccattgata tttaagttaa taaacggtct tcaatttctc aagtttcagt
      541 ttcatttttc ttgttctatt acaacttttt ttacttcttg ttcattagaa aggatctaat
      601 tatctagagc aggagactgc tcaacacaaa actcgagaac atatgtctaa aggtgaagaa
      661 ttattcactg gtgttgtccc aattttggtt gaattagatg gtgatgttaa tggtcacaaa
      721 ttttctgtct ccggtgaagg tgaaggtgat gctacttacg gtaaattgac cttaaaatta
      781 atttgtacta ctggtaaatt gccagttcca tggccaacct tagtcactac tttaggttat
      841 ggtttaatgt gttttgctag atacccagat catatgaaac aacatgactt tttcaagtct
      901 gccatgccag aaggttatgt tcaagaaaga actatttttt tcaaagatga cggtaactac
      961 aagaccagag ctgaagtcaa gtttgaaggt gataccttag ttaatagaat cgaattaaaa
     1021 ggtattgatt ttaaagaaga tggtaacatt ttaggtcaca aattggaata caactataac
     1081 tctcacaatg tttacatcac tgctgacaaa caaaagaatg gtatcaaagc taacttcaaa
     1141 attagacaca acattgaaga tggtggtgtt caattagctg accattatca acaaaatact
     1201 ccaattggtg atggtccagt cttgttacca gacaaccatt acttatccta tcaatctgcc
     1261 ttatccaaag atccaaacga aaagagagac cacatggtct tgttagaatt tgttacttaa
     1321 tctagaaatt atctaggatc caggcagaga aaggtcgata cggacggaat gtgatggcct
     1381 ggatccaaca caaaactcga gaacatcccg ggaaaatcta gaattccttc gatagatcaa
     1441 tttttttctt ttctctttcc ccatccttta cgctaaaata atagtttatt ttattttttg
     1501 aatatttttt atttatatac gtatatatag actattattt atcttttaat gattattaag
     1561 atttttatta aaaaaaaatt cgctcctctt ttaatgcctt tatgcagttt ttttttccca
     1621 ttcgatattt ctatgttcgg gttcagcgta ttttaagttt aataactcga aaattctgcg
     1681 ttcgttaaag cttggcgtaa tcatggtcat agctgttact ggccgtcgtt ttacaacgtc
     1741 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     1801 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     1861 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     1921 accgcatata tcgctgggcc attctcatga agaatatctt gaatttattg tcatattact
     1981 agttggtgtg gaagtccata tatcggtgat caatatagtg gttgacatgc tggctagtca
     2041 acattgagcc ttttgatcat gcaaatatat tacggtattt tacaatcaaa tatcaaactt
     2101 aactattgac tttataactt atttaggtgg taacattctt ataaaaaaga aaaaaattac
     2161 tgcaaaacag tactagcttt taacttgtat cctaggttat ctatgctgtc tcaccataga
     2221 gaatattacc tatttcagaa tgtatgtcca tgattcgccg ggtaaataca tataatacac
     2281 aaatctggct taataaagtc tataatatat ctcataaaga agtgctaaat tggctagtgc
     2341 tatatatttt taagaaaatt tcttttgact aagtccatat cgactttgta aaagttcact
     2401 ttagcataca tatattacac gagccagaaa ttgtaacttt tgcctaaaat cacaaattgc
     2461 aaaatttaat tgcttgcaaa aggtcacatg cttataatca acttttttaa aaatttaaaa
     2521 tactttttta ttttttattt ttaaacataa atgaaataat ttatttattg tttatgatta
     2581 ccgaaacata aaacctgctc aagaaaaaga aactgttttg tccttggaaa aaaagcacta
     2641 cctaggagcg gccaaaatgc cgaggctttc atagcttaaa ctctttacag aaaataggca
     2701 ttatagatca gttcgagttt tcttattctt ccttccggtt ttatcgtcac agttttacag
     2761 taaataagta tcacctctta gagttcgatg ataagctgtc aaacatgaga attaattcca
     2821 catgttaaaa tagtgaagga gcatgttcgg cacacagtgg accgaacgtg gggtaagtgc
     2881 actagggtcc ggttaaacgg atctcgcatt gatgaggcaa cgctaattat caacatatag
     2941 attgttatct atctgcatga acacgaaatc tttacttgac gacttgaggc tgatggtgtt
     3001 tatgcaaaga aaccactgtg tttaatatgt gtcactgttt gatattactg tcagcgtaga
     3061 agataatagt aaaagcggtt aataagtgta tttgagataa gtgtgataaa gtttttacag
     3121 cgaaaagacg ataaatacaa gaaaatgatt acgaggatac ggagagaggt atgtacatgt
     3181 gtatttatat actaagctgc cggcggttgt ttgcaagacc gagaaaaggc tagcaagaat
     3241 cgggtcattg tagcgtatgc gcctgtgaac attctcttca acaagtttga ttccattgcg
     3301 gtgaaatggt aaaagtcaac cccctgcgat gtatattttc ctgtacaatc aatcaaaaag
     3361 ccaaatgatt tagcattatc tttacatctt gttattttac agattttatg tttagatctt
     3421 ttatgcttgc ttttcaaaag gcttgcaggc aagtgcacaa acaatactta aataaatact
     3481 actcagtaat aacctatttc ttagcatttt tgacgaaatt tgctattttg ttagagtctt
     3541 ttacaccatt tgtctccaca cctccgctta catcaacacc aataacgcca tttaatctaa
     3601 gcgcatcacc aacattttct ggcgtcagtc caccagctaa cataaaatgt aagctctcgg
     3661 ggctctcttg ccttccaacc cagtcagaaa tcgagttcca atccaaaagt tcacctgtcc
     3721 cacctgcttc tgaatcaaac aagggaataa acgaatgagg tttctgtgaa gctgcactga
     3781 gtagtatgtt gcagtctttt ggaaatacga gtcttttaat aactggcaaa ccgaggaact
     3841 cttggtattc ttgccacgac tcatctccat gcagttggac gatatcaatg ccgtaatcat
     3901 tgaccagagc caaaacatcc tccttaggtt gattacgaaa cacgccaacc aagtatttcg
     3961 gagtgcctga actattttta tatgctttta caagacttga aattttcctt gcaataaccg
     4021 ggtcaattgt tctctttcta ttgggcacac atataatacc cagcaagtca gcatcggaat
     4081 ctagtgcaca ttctgcggcc tctgtgctct gcaagccgca aactttcacc aatggaccag
     4141 aactacctgt gaaattaata acagacatac tccaagctgc ctttgtgtgc ttaatcacgt
     4201 atactcacgt gctcaatagt caccaatgcc ctccctcttg gccctctcct tttctttttt
     4261 cgaccgaatt aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     4321 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     4381 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4441 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     4501 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4561 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4621 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4681 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     4741 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4801 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4861 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4921 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4981 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     5041 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     5101 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     5161 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     5221 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     5281 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     5341 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     5401 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     5461 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5521 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5581 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5641 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5701 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5761 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5821 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5881 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5941 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6001 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6061 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6121 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     6181 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     6241 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     6301 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     6361 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     6421 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     6481 tcacacagga aacagctatg accatgatta cgcc
//
